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Characterization of Nonribosomal Peptide Synthetases with NRPSsp

  • Carlos PrietoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1401)

Abstract

Bioinformatic sequence analysis allows the functional characterization of newly sequenced proteins. Nonribosomal peptide synthetases (NRPSs) are multi-modular enzymes involved in the biosynthesis of natural products. The current omics era has enabled the exponential growth of the sequenced NRPS, and it is important to characterize the final product of these synthetases. Here, how to achieve the prediction of substrates which bind to adenylation domains in NRPS with NRPSsp (www.nrpssp.com) bioinformatic tool is described.

Key words

Nonribosomal Peptide Synthetases Bioinformatics Classifier NRPSsp 

Notes

Acknowledgments

This work was supported by Agencia de Inversiones y Servicios de Castilla y León (record CCTT/10/LE/0001) and by Juan de la Cierva programme (JCI-2009-05444) of the Ministry of Science and Innovation (Spain).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Bioinformatics Service, Nucleus, University of Salamanca (USAL)SalamancaSpain

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