Environmental Responses in Plants pp 133-140

Part of the Methods in Molecular Biology book series (MIMB, volume 1398) | Cite as

Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock

Protocol

Abstract

Circadian clocks drive 24 h biological rhythms to optimize physiology and development in response to the rotation of the planet. In plants, photosynthesis is modulated by the circadian clock and contributes to daily rhythms in cellular metabolism. In addition to light and temperature, sugar produced from photosynthesis acts as a zeitgeber to contribute to setting of the plant circadian clock. Here, we describe methods to manipulate photosynthetic output and sugar availability in Arabidopsis seedlings. These protocols have been applied to investigate the effects on the Arabidopsis circadian network, but are easily adaptable to other processes in plants.

Key words

Arabidopsis Circadian clock Photosynthesis Sugars Luciferase CO2-free air DCMU 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.School of BiosciencesThe University of MelbourneParkvilleAustralia
  2. 2.Department of Plant SciencesUniversity of CambridgeCambridgeUK

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