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Biopsychronology: A Method Using Live Tissue Staining to Image Cell Function in the Kidney

  • Muhammad Imtiaz Ashraf
  • Dietmar Fries
  • Werner Streif
  • Felix Aigner
  • Paul Hengster
  • Jakob Troppmair
  • Martin Hermann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1397)

Abstract

Methods to monitor the status of a graft prior to transplantation are highly desirable to avoid unnecessary surgical interventions and follow-up treatments and to optimize the clinical outcome as delayed graft function may lead to costly and lengthy follow-up treatments or even organ loss. As a promising step in this direction we present a method which combines the use of fine needle biopsies, the staining of living cells with dyes suitable to monitor mitochondrial status/cellular integrity, and live confocal real-time analysis.

This approach provides information about the functional and structural intactness of an organ within a few minutes. To confirm the feasibility of this approach, we recently published a pilot study using rodent kidneys. The results demonstrated that this method is suitable to monitor organ damage caused by ischemia or short periods of reperfusion. This procedure required minimal time for sample preparation and data acquisition and is suitable for recording damage resulting from unphysiological stress to the organ.

Key words

Biopsy Kidney Real-time live confocal microscopy 

References

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Muhammad Imtiaz Ashraf
    • 1
  • Dietmar Fries
    • 2
  • Werner Streif
    • 3
  • Felix Aigner
    • 1
  • Paul Hengster
    • 1
  • Jakob Troppmair
    • 1
  • Martin Hermann
    • 2
    • 4
  1. 1.Daniel Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Center of Operative MedicineMedical University InnsbruckInnsbruckAustria
  2. 2.Department of General and Surgical Critical Care MedicineMedical University InnsbruckInnsbruckAustria
  3. 3.Department of Pediatrics IMedical University InnsbruckInnsbruckAustria
  4. 4.Department of Anesthesiology and Critical Care MedicineMedical University InnsbruckInnsbruckAustria

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