Superantigens pp 115-124

Part of the Methods in Molecular Biology book series (MIMB, volume 1396)

A Sublethal Swine Model for Defining In Vivo Superantigen-Induced Responses Following Exposure to Staphylococcal Enterotoxin B

  • Kenneth L. Bost
  • Kenneth J. Piller
  • Jack Odle
  • Chad H. Stahl
Protocol

Abstract

In vivo responses to bacterially derived superantigen-like toxins have been difficult to define due to the inherent limitations with rodent models and the relevance that the results obtained from such models may, or may not, have for human pathophysiology. Further the use of challenge doses of superantigen toxins that are lethal or supra-lethal complicates analogies to human exposures which are rarely fatal. Here, we utilize the superantigen, staphylococcal enterotoxin B, at doses that are sublethal in a swine model of toxin-induced incapacitation. Relevant dosing using an animal species for which this toxin is a true superantigen distinguishes this model.

Key words

In vivo swine model Superantigen Sublethal challenge Staphylococcal enterotoxin B Cytokine analyses 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Kenneth L. Bost
    • 1
  • Kenneth J. Piller
    • 1
  • Jack Odle
    • 2
  • Chad H. Stahl
    • 2
  1. 1.Department of Biological SciencesUniversity of North Carolina at CharlotteCharlotteUSA
  2. 2.Department of Animal ScienceNorth Carolina State UniversityRaleighUSA

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