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Superantigens

Volume 1396 of the series Methods in Molecular Biology pp 115-124

Date:

A Sublethal Swine Model for Defining In Vivo Superantigen-Induced Responses Following Exposure to Staphylococcal Enterotoxin B

  • Kenneth L. BostAffiliated withDepartment of Biological Sciences, University of North Carolina at Charlotte Email author 
  • , Kenneth J. PillerAffiliated withDepartment of Biological Sciences, University of North Carolina at Charlotte
  • , Jack OdleAffiliated withDepartment of Animal Science, North Carolina State University
  • , Chad H. StahlAffiliated withDepartment of Animal Science, North Carolina State University

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Abstract

In vivo responses to bacterially derived superantigen-like toxins have been difficult to define due to the inherent limitations with rodent models and the relevance that the results obtained from such models may, or may not, have for human pathophysiology. Further the use of challenge doses of superantigen toxins that are lethal or supra-lethal complicates analogies to human exposures which are rarely fatal. Here, we utilize the superantigen, staphylococcal enterotoxin B, at doses that are sublethal in a swine model of toxin-induced incapacitation. Relevant dosing using an animal species for which this toxin is a true superantigen distinguishes this model.

Key words

In vivo swine model Superantigen Sublethal challenge Staphylococcal enterotoxin B Cytokine analyses