Protocol

Proteomics in Systems Biology

Volume 1394 of the series Methods in Molecular Biology pp 287-310

Date:

Systemic Analysis of Regulated Functional Networks

  • Luis Francisco Hernández SánchezAffiliated withGraduate Program in Optimization, Universidad Autónoma Metropolitana Azcapotzalco
  • , Elise AasebøAffiliated withProteomics Unit, Department of Biomedicine, University of Bergen
  • , Frode SelheimAffiliated withProteomics Unit, Department of Biomedicine, University of Bergen
  • , Frode S. BervenAffiliated withProteomics Unit, Department of Biomedicine, University of BergenKG Jebsen Centre for Multiple Sclerosis Research, Department of Clinical Medicine, University of BergenNorwegian Multiple Sclerosis Competence Centre, Department of Neurology, Haukeland University Hospital
  • , Helge RæderAffiliated withKG Jebsen Center for Diabetes Research, Department of Clinical Science, University of BergenDepartment of Pediatrics, Haukeland University Hospital
  • , Harald BarsnesAffiliated withProteomics Unit, Department of Biomedicine, University of BergenKG Jebsen Center for Diabetes Research, Department of Clinical Science, University of Bergen
  • , Marc VaudelAffiliated withProteomics Unit, Department of Biomedicine, University of Bergen Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

In biological and medical sciences, high throughput analytical methods are now commonly used to investigate samples of different conditions, e.g., patients versus controls. Systemic functional analyses emerged as a reference method to go beyond a list of regulated compounds, and identify activated or inactivated biological functions. This approach holds the promise for a better understanding of biological systems, of the mechanisms involved in disease progression, and thus improved diagnosis, prognosis, and treatment. In this chapter, we present a simple workflow to conduct pathway analyses on biological data using the freely available Reactome platform (http://​www.​reactome.​org).

Key words

Pathway analysis Data interpretation Functional proteomics