Systemic Analysis of Regulated Functional Networks

  • Luis Francisco Hernández Sánchez
  • Elise Aasebø
  • Frode Selheim
  • Frode S. Berven
  • Helge Ræder
  • Harald Barsnes
  • Marc VaudelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1394)


In biological and medical sciences, high throughput analytical methods are now commonly used to investigate samples of different conditions, e.g., patients versus controls. Systemic functional analyses emerged as a reference method to go beyond a list of regulated compounds, and identify activated or inactivated biological functions. This approach holds the promise for a better understanding of biological systems, of the mechanisms involved in disease progression, and thus improved diagnosis, prognosis, and treatment. In this chapter, we present a simple workflow to conduct pathway analyses on biological data using the freely available Reactome platform (

Key words

Pathway analysis Data interpretation Functional proteomics 



F.S.B. and F.S. acknowledge support from the Norwegian Cancer Society. H.B. is supported by the Research Council of Norway.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Luis Francisco Hernández Sánchez
    • 1
  • Elise Aasebø
    • 2
  • Frode Selheim
    • 2
  • Frode S. Berven
    • 2
    • 3
    • 4
  • Helge Ræder
    • 5
    • 6
  • Harald Barsnes
    • 2
    • 5
  • Marc Vaudel
    • 2
    Email author
  1. 1.Graduate Program in OptimizationUniversidad Autónoma Metropolitana AzcapotzalcoMexico CityMexico
  2. 2.Proteomics Unit, Department of BiomedicineUniversity of BergenBergenNorway
  3. 3.KG Jebsen Centre for Multiple Sclerosis Research, Department of Clinical MedicineUniversity of BergenBergenNorway
  4. 4.Norwegian Multiple Sclerosis Competence Centre, Department of NeurologyHaukeland University HospitalBergenNorway
  5. 5.KG Jebsen Center for Diabetes Research, Department of Clinical ScienceUniversity of BergenBergenNorway
  6. 6.Department of PediatricsHaukeland University HospitalBergenNorway

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