Protocols for In Vitro Propagation, Conservation, Synthetic Seed Production, Embryo Rescue, Microrhizome Production, Molecular Profiling, and Genetic Transformation in Ginger (Zingiber officinale Roscoe.)

  • K. Nirmal BabuEmail author
  • K. Samsudeen
  • Minoo Divakaran
  • Geetha S. Pillai
  • V. Sumathi
  • K. Praveen
  • P. N. Ravindran
  • K. V. Peter
Part of the Methods in Molecular Biology book series (MIMB, volume 1391)


Ginger is a rhizomatous plant that belongs to the family Zingiberaceae. It is a herbaceous perennial but cultivated as annual, with crop duration of 7–10 months. Ginger is native to India and Tropical South Asia. The tuberous rhizomes or underground stems of ginger are used as condiment, an aromatic stimulant, and food preservative as well as in traditional medicine. Ginger is propagated vegetatively with rhizome bits as seed material. Cultivation of ginger is plagued by rhizome rot diseases, most of which are mainly spread through infected seed rhizomes. Micropropagation will help in production of disease-free planting material. Sexual reproduction is absent in ginger, making recombinant breeding very impossible. In vitro technology can thus become the preferred choice as it can be utilized for multiplication, conservation of genetic resources, generating variability, gene transfer, molecular tagging, and their utility in crop improvement of these crops.

Key words

Anther culture Artificial/synthetic seeds Cryopreservation Embryo rescue In vitro conservation Micropropagation Molecular profiling Plant regeneration Protoplast isolation Somaclonal variation Somatic embryogenesis Transgenics Ginger Zingiber officinale 



Benzyl adenine


Indole-3-butyric acid




α-Naphthalene acetic acid


Murashige and Skoog


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • K. Nirmal Babu
    • 1
    Email author
  • K. Samsudeen
    • 2
  • Minoo Divakaran
    • 3
  • Geetha S. Pillai
    • 4
  • V. Sumathi
    • 1
  • K. Praveen
    • 1
  • P. N. Ravindran
    • 1
  • K. V. Peter
    • 5
  1. 1.All India Coordinated Research Project on SpicesIndian Institute of Spices ResearchKozhikodeIndia
  2. 2.Central Plantation Crops Research InstituteKasaragodIndia
  3. 3.Providence Women’s CollegeKozhikodeIndia
  4. 4.Centre for Medicinal Plants Research, Arya Vaidya SalaKottakkalIndia
  5. 5.World Noni Research FoundationChennaiIndia

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