Quantitation of Chromosome Damage by Imaging Flow Cytometry

  • Lindsay A. Beaton-GreenEmail author
  • Ruth C. Wilkins
Part of the Methods in Molecular Biology book series (MIMB, volume 1389)


Biodosimetry is a method for measuring the dose of radiation to individuals using biological markers such as chromosome damage. Following mass casualty events, it is important to provide this information rapidly in order to assist with the medical management of potentially exposed casualties. Currently, the gold standard for biodosimetry is the dicentric chromosome assay, which accurately estimates the dose from the number of dicentric chromosomes in lymphocytes. To increase throughput of analysis following a large-scale mass casualty event, this assay has been adapted for use on the imaging flow cytometer. This chapter describes the methods for the identification and quantification of mono- and multicentric chromosomes using the imaging flow cytometer.

Key words

Dicentric chromosome assay Imaging flow cytometry Lymphocytes Chromosome damage Biodosimetry 



The authors gratefully acknowledge the help of Catherine Ferrarotto for help with the sample preparation. This equipment was purchased through a Canadian Safety and Security Program Technology Acquisition Project.


  1. 1.
    International Organization for Standardization (2008) Radiation protection—performance criteria for laboratories performing cytogenetic triage for assessment of mass casualties in radiological or nuclear emergencies—general principles and application to dicentric assay. Geneva: ISO, ISO 21243Google Scholar
  2. 2.
    International Organization for Standardization (2004) Radiation protection—perfomance criteria for service laboratories performing biological dosimetry by cytogenetics. Geneva: ISO, ISO 19238Google Scholar
  3. 3.
    IAEA (2011) Cytogenetic dosimetry: applications in preparedness for and response to radiation emergencies. IAEA, EPR-Biodose, ViennaGoogle Scholar
  4. 4.
    Lloyd DC, Edwards AA, Moquet JE, Guerrero-Carbajal YC (2000) The role of cytogenetics in early triage of radiation casualties. Appl Radiat Isot 52:1107–1112CrossRefPubMedGoogle Scholar
  5. 5.
    Voisin P, Benderitter M, Claraz M, Chambrette V, Sorokine-Durm I, Delbos M, Durand V, Leroy A, Paillole N (2001) The cytogenetic dosimetry of recent accidental overexposure. Cell Mol Biol 47:557–564PubMedGoogle Scholar
  6. 6.
    Flegal FN, Devantier Y, Marro L, Wilkins RC (2012) Validation of QuickScan dicentric chromosome analysis for high throughput radiation biological dosimetry. Health Phys 102:143–153. doi: 10.1097/HP.0b013e3182307758 CrossRefPubMedGoogle Scholar
  7. 7.
    Vaurijoux A, Gruel G, Pouzoulet F, Gregoire E, Martin C, Roch-Lefevre S, Voisin P, Voisin P, Roy L (2009) Strategy for population triage based on dicentric analysis. Radiat Res 171:541–548. doi: 10.1667/RR1664.1 CrossRefPubMedGoogle Scholar
  8. 8.
    Vaurijoux A, Gregoire E, Roch-Lefevre S, Voisin P, Martin C, Voisin P, Roy L, Gruel G (2012) Detection of partial-body exposure to ionizing radiation by the automatic detection of dicentrics. Radiat Res 178:357–364. doi: 10.1667/RR2728.1, CrossRefPubMedGoogle Scholar
  9. 9.
    Fantes JA, Green DK, Elder JK, Malloy P, Evans HJ (1983) Detecting radiation damage to human chromosomes by flow cytometry. Mutat Res 119:161–168CrossRefPubMedGoogle Scholar
  10. 10.
    Fantes JA, Green DK, Malloy P, Sumner AT (1989) Flow cytometry measurements of human chromosome kinetochore labeling. Cytometry 10:134–142CrossRefPubMedGoogle Scholar
  11. 11.
    Brind’amour J, Lansdorp PM (2011) Analysis of repetitive DNA in chromosomes by flow cytometry. Nat Methods 8:484–486. doi: 10.1038/nmeth.1601 CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Sillar R, Young BD (1981) A new method for the preparation of metaphase chromosomes for flow analysis. J Histochem Cytochem 29:74–78CrossRefPubMedGoogle Scholar
  13. 13.
    Beaton LA, Ferrarotto C, Kutzner BC, McNamee JP, Bellie PV, Wilkins RC (2013) Analysis of chromosome damage for biodosimetry using imaging flow cytometry. Mutat Res 756:192–195. doi: 10.1016/j.mrgentox.2013.04.002 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Radiobiology, Consumer and Clinical Radiation Protection BureauHealth CanadaOttawaCanada

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