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Antibody-Mediated Pathogen Resistance in Plants

  • Dieter Peschen
  • Stefan Schillberg
  • Rainer Fischer
Part of the Methods in Molecular Biology book series (MIMB, volume 1385)

Abstract

The methods described in this chapter were developed in order to produce transgenic plants expressing pathogen-specific single-chain variable fragment (scFv) antibodies fused to antifungal peptides (AFPs), conferring resistance against fungal pathogens. We describe the selection from a phage display library of avian scFv antibodies that recognize cell surface proteins on fungi from the genus Fusarium, and the construction of scFv–AFP fusion protein constructs followed by their transient expression in tobacco (Nicotiana spp.) plants and stable expression in Arabidopsis thaliana plants. Using these techniques, the antibody fusion with the most promising in vitro activity can be used to generate transgenic plants that are resistant to pathogens such as Fusarium oxysporum f. sp. matthiolae.

Keywords

Transgenic plants Antibody Antibody fragments Single-chain variable fragment scFv Pathogen Fungal antigens Antifungal peptide Arabidopsis thaliana Fusarium spp. 

Notes

Acknowledgements

We would like to thank all our colleagues at the Fraunhofer IME and the Institute of Biology VII, RWTH Aachen, for their cooperation and helpful discussions. Special thanks to Drs. Yu-Cai Liao and Simone Dorfmüller for their collaboration with the Fusarium work. We would like to thank Dr. Richard M. Twyman for help with the preparation of this manuscript.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Dieter Peschen
    • 1
  • Stefan Schillberg
    • 1
  • Rainer Fischer
    • 1
  1. 1.Fraunhofer Institute for Molecular Biology and Applied EcologyAachenGermany

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