Standardized Multi-Color Flow Cytometry and Computational Biomarker Discovery

  • Stephan Schlickeiser
  • Mathias Streitz
  • Birgit SawitzkiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1371)


Multi-color flow cytometry has become a valuable and highly informative tool for diagnosis and therapeutic monitoring of patients with immune deficiencies or inflammatory disorders. However, the method complexity and error-prone conventional manual data analysis often result in a high variability between different analysts and research laboratories. Here, we provide strategies and guidelines aiming at a more standardized multi-color flow cytometric staining and unsupervised data analysis for whole blood patient samples.

Key words

Flow cytometry Immune monitoring Standardization Data analysis 



This work was supported by the EU FP7 grant “The ONE Study” and the EU COST initiative “A-FACCT” provided to Birgit Sawitzki.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Stephan Schlickeiser
    • 1
  • Mathias Streitz
    • 1
  • Birgit Sawitzki
    • 1
    Email author
  1. 1.Institute of Medical ImmunologyCharité University MedicineBerlinGermany

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