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Microarray Technology pp 249–271Cite as

Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays)

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Part of the Methods in Molecular Biology book series (MIMB,volume 1368)

Abstract

Screening of peptides to find the ligands that bind to specific targets is an important step in drug discovery. These high-throughput screens require large number of structural variants of peptides to be synthesized and tested. This chapter describes the generation of arrays of peptides on Teflon-patterned sheets of paper. First, the protocol describes the patterning of paper with a Teflon solution to produce arrays with solvophobic barriers that are able to confine organic solvents. Next, we describe the parallel syntheses of 96 peptides on Teflon-patterned arrays using the SPOT synthesis method.

Key words

  • Peptides
  • Paper array
  • Paper patterning
  • SPOT synthesis
  • Ligand screening

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  • DOI: 10.1007/978-1-4939-3136-1_18
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Author information

Authors and Affiliations

  1. Department of Chemistry, Alberta Glycomics Center, University of Alberta, 11227 Saskatchewan Drive, Edmonton, AB, Canada, T6G 2G2

    Frédérique Deiss, Yang Yang & Ratmir Derda

Authors
  1. Frédérique Deiss
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  2. Yang Yang
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  3. Ratmir Derda
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Corresponding author

Correspondence to Ratmir Derda .

Editor information

Editors and Affiliations

  1. Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    Paul C.H. Li

  2. Dept. of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada

    Abootaleb Sedighi

  3. Department of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada

    Lin Wang

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Deiss, F., Yang, Y., Derda, R. (2016). Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays). In: Li, P., Sedighi, A., Wang, L. (eds) Microarray Technology. Methods in Molecular Biology, vol 1368. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3136-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3136-1_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3135-4

  • Online ISBN: 978-1-4939-3136-1

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