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Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays)

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Part of the Methods in Molecular Biology book series (MIMB,volume 1368)

Abstract

Screening of peptides to find the ligands that bind to specific targets is an important step in drug discovery. These high-throughput screens require large number of structural variants of peptides to be synthesized and tested. This chapter describes the generation of arrays of peptides on Teflon-patterned sheets of paper. First, the protocol describes the patterning of paper with a Teflon solution to produce arrays with solvophobic barriers that are able to confine organic solvents. Next, we describe the parallel syntheses of 96 peptides on Teflon-patterned arrays using the SPOT synthesis method.

Key words

  • Peptides
  • Paper array
  • Paper patterning
  • SPOT synthesis
  • Ligand screening

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Correspondence to Ratmir Derda .

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Deiss, F., Yang, Y., Derda, R. (2016). Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays). In: Li, P., Sedighi, A., Wang, L. (eds) Microarray Technology. Methods in Molecular Biology, vol 1368. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3136-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3136-1_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3135-4

  • Online ISBN: 978-1-4939-3136-1

  • eBook Packages: Springer Protocols