Protocol

Microarray Technology

Volume 1368 of the series Methods in Molecular Biology pp 249-271

Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays)

  • Frédérique DeissAffiliated withDepartment of Chemistry, Alberta Glycomics Center, University of Alberta
  • , Yang YangAffiliated withDepartment of Chemistry, Alberta Glycomics Center, University of Alberta
  • , Ratmir DerdaAffiliated withDepartment of Chemistry, Alberta Glycomics Center, University of Alberta Email author 

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Abstract

Screening of peptides to find the ligands that bind to specific targets is an important step in drug discovery. These high-throughput screens require large number of structural variants of peptides to be synthesized and tested. This chapter describes the generation of arrays of peptides on Teflon-patterned sheets of paper. First, the protocol describes the patterning of paper with a Teflon solution to produce arrays with solvophobic barriers that are able to confine organic solvents. Next, we describe the parallel syntheses of 96 peptides on Teflon-patterned arrays using the SPOT synthesis method.

Key words

Peptides Paper array Paper patterning SPOT synthesis Ligand screening