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DNA Microarray-Based Diagnostics

  • Mahsa Gharibi Marzancola
  • Abootaleb Sedighi
  • Paul C. H. Li
Part of the Methods in Molecular Biology book series (MIMB, volume 1368)

Abstract

The DNA microarray technology is currently a useful biomedical tool which has been developed for a variety of diagnostic applications. However, the development pathway has not been smooth and the technology has faced some challenges. The reliability of the microarray data and also the clinical utility of the results in the early days were criticized. These criticisms added to the severe competition from other techniques, such as next-generation sequencing (NGS), impacting the growth of microarray-based tests in the molecular diagnostic market.

Thanks to the advances in the underlying technologies as well as the tremendous effort offered by the research community and commercial vendors, these challenges have mostly been addressed. Nowadays, the microarray platform has achieved sufficient standardization and method validation as well as efficient probe printing, liquid handling and signal visualization. Integration of various steps of the microarray assay into a harmonized and miniaturized handheld lab-on-a-chip (LOC) device has been a goal for the microarray community. In this respect, notable progress has been achieved in coupling the DNA microarray with the liquid manipulation microsystem as well as the supporting subsystem that will generate the stand-alone LOC device.

In this chapter, we discuss the major challenges that microarray technology has faced in its almost two decades of development and also describe the solutions to overcome the challenges. In addition, we review the advancements of the technology, especially the progress toward developing the LOC devices for DNA diagnostic applications.

Key words

DNA microarray Stand-alone lab-on-a-chip (LOC) device Diagnostic tool Microfluidics Label-free detection Nanoarrays 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Mahsa Gharibi Marzancola
    • 1
  • Abootaleb Sedighi
    • 1
  • Paul C. H. Li
    • 1
  1. 1.Department of ChemistrySimon Fraser UniversityBurnabyCanada

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