Oriented Immobilized Sialyloligo-macroligand Microarray
Silaic acid is the most common terminal glycan on cell surface glycoproteins and glycolipids, involving in many biological processes. Studying interactions between multivalent sialic acid scaffolds and proteins binding to it will give incredible information in understanding the biological process the sialic acid is involved in. Here we describe chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.
Key wordsSialic acid Glycan array Glycopolymer Lectin Hemagglutinin SPR
This work was supported from AHA Grant-in Aid grant, Ohio Research Scholars Program grant, NSF MRI grant, and Doctoral Dissertation Research Expense Award Program (Cleveland State University). Thanks to Dr. Dale Ray at Case NMR Center for NMR studies.
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