Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry

Part of the Methods in Molecular Biology book series (MIMB, volume 1363)

Abstract

Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens.

Key words

VIGS Silencing Reverse genetics TRV Tomato 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Trisaia Research CenterENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development)RotondellaItaly
  2. 2.Casaccia Research CenterENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development)RomeItaly

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