Protocol

Plant Signal Transduction

Volume 1363 of the series Methods in Molecular Biology pp 65-78

Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry

  • Elio FantiniAffiliated withTrisaia Research Center, ENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development) Email author 
  • , Giovanni GiulianoAffiliated withCasaccia Research Center, ENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development) Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens.

Key words

VIGS Silencing Reverse genetics TRV Tomato