Protocol

Plant Signal Transduction

Volume 1363 of the series Methods in Molecular Biology pp 47-55

Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation

  • Masahito ShikataAffiliated withFaculty of Life and Environmental Sciences, University of Tsukuba
  • , Hiroshi EzuraAffiliated withFaculty of Life and Environmental Sciences, University of Tsukuba Email author 

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Abstract

Tomato is a model plant for fruit development, a unique feature that classical model plants such as Arabidopsis and rice do not have. The tomato genome was sequenced in 2012 and tomato is becoming very popular as an alternative system for plant research. Among many varieties of tomato, Micro-Tom has been recognized as a model cultivar for tomato research because it shares some key advantages with Arabidopsis including its small size, short life cycle, and capacity to grow under fluorescent lights at a high density.

Mutants and transgenic plants are essential materials for functional genomics research, and therefore, the availability of mutant resources and methods for genetic transformation are key tools to facilitate tomato research. Here, we introduce the Micro-Tom mutant database “TOMATOMA” and an efficient transformation protocol for Micro-Tom.

Key words

Tomato Micro-Tom Mutant Resource Database Transformation Agrobacterium