Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation

Part of the Methods in Molecular Biology book series (MIMB, volume 1363)

Abstract

Tomato is a model plant for fruit development, a unique feature that classical model plants such as Arabidopsis and rice do not have. The tomato genome was sequenced in 2012 and tomato is becoming very popular as an alternative system for plant research. Among many varieties of tomato, Micro-Tom has been recognized as a model cultivar for tomato research because it shares some key advantages with Arabidopsis including its small size, short life cycle, and capacity to grow under fluorescent lights at a high density.

Mutants and transgenic plants are essential materials for functional genomics research, and therefore, the availability of mutant resources and methods for genetic transformation are key tools to facilitate tomato research. Here, we introduce the Micro-Tom mutant database “TOMATOMA” and an efficient transformation protocol for Micro-Tom.

Key words

Tomato Micro-Tom Mutant Resource Database Transformation Agrobacterium 

References

  1. 1.
    The Tomato Genome Consortium (2012) The tomato genome sequence provides insights into fleshy fruit evolution. Nature 485:635–641CrossRefGoogle Scholar
  2. 2.
    Ariizumi T, Aoki K, Ezura H (2011) Systematic development of tomato bioresources in Japan. Interdiscip Bio Central 3:1–7CrossRefGoogle Scholar
  3. 3.
    Saito T, Ariizumi T, Okabe Y, Asamizu E, Hiwasa-Tanase K, Fukuda N, Mizoguchi T, Yamazaki Y, Aoki K, Ezura H (2011) TOMATOMA: a novel tomato mutant database distributing Micro-Tom mutant collections. Plant Cell Physiol 52:283–296PubMedCentralCrossRefPubMedGoogle Scholar
  4. 4.
    Sun HJ, Uchii S, Watanabe S, Ezura H (2006) A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics. Plant Cell Physiol 47:426–431CrossRefPubMedGoogle Scholar
  5. 5.
    Watanabe S, Mizoguchi T, Aoki K, Kubo Y, Mori H, Imanishi S, Yamazaki Y, Shibata D, Ezura H (2007) Ethylmethanesulfonate (EMS) mutagenesis of Solunum lycopercisum cv. Micro-Tom for large-scale mutant screens. Plant Biotech 24:33–38CrossRefGoogle Scholar
  6. 6.
    Matsukura C, Yamaguchi I, Inamura M, Ban Y, Kobayashi Y, Yin YG, Saito T, Kuwata C, Imanishi S, Nishimura S (2007) Generation of gamma irradiation-induced mutant lines of the miniature tomato (Solanum lycopersicum L.) cultivar ‘Micro-Tom’. Plant Biotech 24:39–44CrossRefGoogle Scholar
  7. 7.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  8. 8.
    Mason G, Provero P, Vaira AM, Accotto GP (2002) Estimating the number of integrations in transformed plants by quantitative real-time PCR. BMC Biotechnol 2:20PubMedCentralCrossRefPubMedGoogle Scholar
  9. 9.
    Bubner B, Baldwin IT (2004) Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Rep 23:263–271CrossRefPubMedGoogle Scholar
  10. 10.
    Eshed Y, Zamir D (1994) A genomic library of Lycopersicon pennellii in L. esculentum: a tool for fine mapping of genes. Euphytica 79:175–179CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Faculty of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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