Experimental Evolution and Resequencing Analysis of Yeast
Experimental evolution of microbes is a powerful tool to study adaptation to strong selection, the mechanism of evolution and the development of new traits. The development of high-throughput sequencing methods has given researchers a new ability to cheaply and easily identify mutations genome wide that are selected during the course of experimental evolution. Here we provide a protocol for conducting experimental evolution of yeast using chemostats, including fitness measurement and whole genome sequencing of evolved clones or populations collected during the experiment. Depending on the number of generations appropriate for the experiment, the number of samples tested and the sequencing platform, this protocol takes from 1 month to several months to be completed, with the possibility of processing several strains or mutants at once.
Key wordsYeast Chemostats Fitness Whole genome sequencing Nextera MiSeq
Thanks to Emily Mitchell and Giang T. Ong for their protocols. This work was supported by grants R01 GM094306 and P41 GM103533 from the National Institute of General Medical Sciences from the National Institutes of Health, and National Science Foundation grant 1120425. MJD is a Rita Allen Foundation Scholar, and a Fellow in the Genetic Networks program at the Canadian Institute for Advanced Research.
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