Profiling of Yeast Lipids by Shotgun Lipidomics
Lipidomics is a rapidly growing technology for identification and quantification of a variety of cellular lipid molecules. Following the successful development and application of functional genomic technologies in yeast Saccharomyces cerevisiae, we witness a recent expansion of lipidomics applications in this model organism. The applications include detailed characterization of the yeast lipidome as well as screening for perturbed lipid phenotypes across hundreds of yeast gene deletion mutants. In this chapter, we describe sample handling, mass spectrometry, and bioinformatics methods developed for yeast lipidomics studies.
Key wordsYeast Lipidomics Mass spectrometry Lipids
The authors would like to thank Kai Simons for critically reading the manuscript. C.K. acknowledges fruitful discussions with Julio L. Sampaio and Michal A. Surma.
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