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Systematic Determination of Transcription Factor DNA-Binding Specificities in Yeast

  • Lourdes Peña-Castillo
  • Gwenael Badis
Part of the Methods in Molecular Biology book series (MIMB, volume 1361)

Abstract

Understanding how genes are regulated, decoding their “regulome”, is one of the main challenges of the post-genomic era. Here, we describe the in vitro method we used to associate cis-regulatory sites with cognate trans-regulators by characterizing the DNA-binding specificity of the vast majority of yeast transcription factors using Protein Binding Microarrays. This approach can be implemented to any given organism.

Key words

Transcription regulation Transcription factors DNA binding domain cis-regulatory element Enhancers Binding sites 

Notes

Acknowledgment

We thank Shaheynoor Talukder for standard operating procedure and Timothy R. Hughes for data availability. We also thank Esther T. Chan for useful comments. G.B. work was supported by the CIHR, the Institut Pasteur and the Centre National pour la Recherche Scientifique. LPC’s work was supported by a NSERC Discovery Grant and Memorial University of Newfoundland.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of BiologyMemorial University of NewfoundlandSt. John’sCanada
  2. 2.Department of Computer ScienceMemorial University of NewfoundlandSt. John’sCanada
  3. 3.Institut Pasteur, Génétique des Interactions MacromoléculairesCentre National de la Recherche ScientifiqueParisFrance

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