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Characterization of an Engineered Src Kinase to Study Src Signaling and Biology

  • Leanna R. Gentry
  • Andrei V. Karginov
  • Klaus M. Hahn
  • Channing J. Der
Part of the Methods in Molecular Biology book series (MIMB, volume 1360)

Abstract

Pharmacologic inhibitors of protein kinases comprise the vast majority of approved signal transduction inhibitors for cancer treatment. An important facet of their clinical development is the identification of the key substrates critical for their driver role in cancer. One approach for substrate identification involves evaluating the phosphorylation events associated with stable expression of an activated protein kinase. Another involves genetic or pharmacologic inhibition of protein kinase expression or activity. However, both approaches are limited by the dynamic nature of signaling, complicating whether phosphorylation changes are primary or secondary activities of kinase function. We have developed rapamycin-regulated (RapR) protein kinases as molecular tools that allow for the study of spatiotemporal regulation of signaling. Here we describe the application of this technology to the Src tyrosine kinase and oncoprotein (RapR-Src). We describe how to achieve stable expression of this tool in cell lines and how to subsequently activate the tool and determine its function in signaling and morphology.

Key words

FK506-binding protein KRAS mTOR Oncogene Pancreatic cancer Rapamycin Src Tyrosine kinase 

Notes

Acknowledgements

This work was supported, in whole or in part, by National Institutes of Health Grants CA042978 to C.J.D. and CA175747 to C. J. D. and K. M. H.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Leanna R. Gentry
    • 1
  • Andrei V. Karginov
    • 2
  • Klaus M. Hahn
    • 1
  • Channing J. Der
    • 1
  1. 1.Department of PharmacologyUniversity of North Carolina at Chapel HillChapel HillUSA
  2. 2.Department of PharmacologyUniversity of Illinois at ChicagoChicagoUSA

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