Advertisement

Somatic Embryogenesis in Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae)

  • Miguel P. GuerraEmail author
  • Neusa Steiner
  • Francine L. Farias-Soares
  • Leila do N. Vieira
  • Hugo P. F. Fraga
  • Gladys D. Rogge-Renner
  • Sara B. Maldonado
Part of the Methods in Molecular Biology book series (MIMB, volume 1359)

Abstract

This chapter deals with the features of somatic embryogenesis (SE) in Araucaria angustifolia, an endangered and native conifer from south Brazil. In this species SE includes the induction and proliferation of embryogenic cultures composed of pro-embryogenic masses (PEMs), which precede somatic embryos development. A. angustifolia SE model encompasses induction, proliferation, pre-maturation, and maturation steps. Double-staining with acetocarmine and Evan’s blue is useful to evaluate the embryonic somatic structures. In this chapter we describe A. angustifolia SE protocols and analyzes morphological features in the different SE developmental stages.

Key words

Conifers Forest biotechnology Germplasm conservation Plant cell culture Plant physiology Somatic embryogenesis 

References

  1. 1.
    Guerra MP, Steiner N, Mantovani A, Nodari RO, Reis MS, Santos KL (2008) Araucária: Evolução, ontogênese e diversidade genética. In: Barbieri RL, Stumpf ERT (eds) Origem e evolução de plantas cultivadas, 1st edn. Embrapa Informação Tecnológica, Brasília, pp 149–184Google Scholar
  2. 2.
    IUCN, IUCN Red List of Threatened Species, Version 2011.2. www.iucnredlist.org, Downloaded on 4.04.2012
  3. 3.
    Stefenon VM, Steiner N, Guerra MP, Nodari RO (2009) Integrating approaches towards the conservation of forest genetic resources: a case study of Araucaria angustifolia. Biodivers Conserv 18:2433–2448CrossRefGoogle Scholar
  4. 4.
    Farias-Soares FL, Burrieza HP, Steiner N, Maldonado S, Guerra MP (2013) Immunoanalysis of dehydrins in Araucaria angustifolia embryos. Protoplasma 250:911–918CrossRefPubMedGoogle Scholar
  5. 5.
    Steiner N, Santa-Catarina C, Andrade JBR, Balbuena TS, Guerra MP, Handro W, Floh EIS, Silveira V (2008) Araucaria angustifolia biotechnology-review. Funct Plant Sci Biotechnol 2:20–28Google Scholar
  6. 6.
    von Arnold S, Sabala I, Bozkov P, Dyachok J, Filonova L (2002) Developmental pathways of somatic embryogenesis. Plant Cell Tiss Organ Cult 69:233–249CrossRefGoogle Scholar
  7. 7.
    Steiner N, Vieira FN, Maldonado S, Guerra MP (2005) Carbon source affects morphogenesis and histodifferentiation of A. angustifolia embryogenic cultures. Braz Arch Biol Technol 48:896–903CrossRefGoogle Scholar
  8. 8.
    Astarita LV, Guerra MP (1998) Early somatic embryogenesis in Araucaria angustifolia- induction and maintenance of embryonal-suspensor mass cultures. Braz J Plant Physiol 10:113–118Google Scholar
  9. 9.
    Santos ALW, Silveira V, Steiner N, Vidor M, Guerra MP (2002) Somatic embryogenesis in Paraná Pine (Araucaria angustifolia (Bert.) O. Kuntze). Braz Arch Biol Technol 45:97–106CrossRefGoogle Scholar
  10. 10.
    Silveira V, Steiner N, Santos ALW, Nodari RO, Guerra MP (2002) Biotechnology tools in Araucaria angustifolia conservation and improvement: inductive factors affecting somatic embryogenesis. Crop Breed Appl Biotechnol 2:463–470CrossRefGoogle Scholar
  11. 11.
    Santos ALW, Steiner N, Guerra MP, Zoglauer K, Moerschbacher BM (2008) Somatic embryogenesis in Araucaria angustifolia. Biol Plant 52:95–99Google Scholar
  12. 12.
    Santos ALW, Silveira V, Steiner N, Maraschin M, Guerra MP (2010) Biochemical and morphological changes during the growth kinetics of Araucaria angustifolia suspension cultures. Braz Arch Biol Technol 53:497–504CrossRefGoogle Scholar
  13. 13.
    Dutra NT, Silveira V, de Azevedo IG, Gomes-Neto LR, Façanha AR, Steiner N, Guerra MP, Floh EIS, Santa-Catarina C (2013) Polyamines affect the cellular growth and structure of pro-embryogenic masses in Araucaria angustifolia embryogenic cultures through the modulation of proton pump activities and endogenous levels of polyamines. Physiol Plant 148:121–132CrossRefPubMedGoogle Scholar
  14. 14.
    Vieira LN, Santa-Catarina C, Fraga HPF, dos Santos ALW, Steinmacher DA, Schlogl PS, Silveira V, Steiner N, Floh EIS, Guerra MP (2012) Glutathione improves early somatic embryogenesis in Araucaria angustifolia (Bert) O. Kuntze by alteration in nitric oxide emission. Plant Sci 195:80–87CrossRefGoogle Scholar
  15. 15.
    Silveira V, Santa-Catarina C, Tun NN, Scherer GFE, Handro W, Guerra MP, Floh EIS (2006) Polyamine effects on the endogenous polyamine contents, nitric oxide release, growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia (Bert.) O. Kuntze. Plant Sci 171:91–98CrossRefGoogle Scholar
  16. 16.
    Gupta PK, Pullman GS (1991) Method for reproducing coniferous plants by somatic embryogenesis using abscisic acid and osmotic potential variation, US patent 5, 36–37Google Scholar
  17. 17.
    Becwar MR, Wann SR, Johnson MA, Verhagen SA, Feirer RP, Nagmani R (1988) Development and characterization of in vitro embryogenic systems in conifers. In: Ahuja MR (ed) Somatic cell genetics of woody plants. Kluwer Academic, Dordrecht, pp 1–18CrossRefGoogle Scholar
  18. 18.
    Jo L, dos Santos ALW, Bueno CA, Barbosa HR, Floh EIS (2013) Proteomic analysis and polyamines, ethylene and reactive oxygen species levels of Araucaria angustifolia (Brazilian pine) embryogenic cultures with different embryogenic potential. Tree Physiol 34:94–104CrossRefPubMedGoogle Scholar
  19. 19.
    Gupta PK, Durzan DJ (1987) Biotechnology of somatic polyembryogenesis and plantlet regeneration in loblolly pine. Nat Biotechnol 5:147–151CrossRefGoogle Scholar
  20. 20.
    Filonova LH, Bozhkov PV, von Arnold S (2000) Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-lapse tracking. J Exp Bot 51:249–264CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Miguel P. Guerra
    • 1
    Email author
  • Neusa Steiner
    • 2
  • Francine L. Farias-Soares
    • 1
  • Leila do N. Vieira
    • 1
  • Hugo P. F. Fraga
    • 1
  • Gladys D. Rogge-Renner
    • 3
    • 4
  • Sara B. Maldonado
    • 5
  1. 1.Graduate Program in Plant Genetic Resources, Plant Developmental Physiology and Genetics LaboratoryFederal University of Santa Catarina (UFSC)FlorianópolisBrazil
  2. 2.Department of BotanyUFSCFlorianópolisBrazil
  3. 3.Department of Cell Biology, Embryology and Genetics, Plant Cell Biology LaboratoryUFSCFlorianópolisBrazil
  4. 4.Department of Biological SciencesUniversity of Joinville RegionJoinvilleBrazil
  5. 5.Department of Biodiversity and Experimental BiologyUniversity of Buenos AiresBuenos AiresArgentina

Personalised recommendations