In Vitro Embryogenesis in Higher Plants pp 329-339 | Cite as
Somatic Embryogenesis Induction and Plant Regeneration in Strawberry Tree (Arbutus unedo L.)
Protocol
Abstract
Somatic embryogenesis is a powerful tool both for cloning and studies of genetic transformation and embryo development. Most protocols for somatic embryogenesis induction start from zygotic embryos or embryonic-derived tissues which do not allow the propagation of elite trees. In the present study, a reliable protocol for somatic embryogenesis induction from adult trees of strawberry tree is described. Leaves from in vitro proliferating shoots were used to induce somatic embryo formation on a medium containing an auxin and a cytokinin. Somatic embryos germinated in a plant growth regulator-free medium.
Key words
Epicormic shoots Ericaceae Fruit Germination In vitro Neglected crops Shoot proliferation Somatic embryoNotes
Acknowledgments
This work was supported by Fundação para a Ciência e Tecnologia (Portugal), research contract PTDC/AGR-FOR/3746/2012, and by a postdoctoral research fellowship (SFRH/BPD/91461/2012) awarded to Sandra Correia.
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