HIV Protocols pp 281-292 | Cite as

Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells

  • Nigora Mukhamedova
  • Beda Brichacek
  • Christina Darwish
  • Anastas Popratiloff
  • Dmitri Sviridov
  • Michael Bukrinsky
Part of the Methods in Molecular Biology book series (MIMB, volume 1354)

Abstract

Cholesterol is an essential component of the cellular membranes and, by extension, of the HIV envelope membrane, which is derived from the host cell plasma membrane. Depletion of the cellular cholesterol has an inhibitory effect on HIV assembly, reduces infectivity of the produced virions, and makes the cell less susceptible to HIV infection. It is not surprising that the virus has evolved to gain access to cellular proteins regulating cholesterol metabolism. One of the key mechanisms used by HIV to maintain high levels of cholesterol in infected cells is Nef-mediated inhibition of cholesterol efflux and the cholesterol transporter responsible for this process, ABCA1. In this chapter, we describe methods to investigate these effects of HIV-1 infection.

Key words

HIV-1 Nef Cholesterol efflux ABCA1 Confocal microscopy Image analysis 

Notes

Acknowledgments

This study was supported by NIH grants R01HL093818, R01HL101274, R21AI108533, P30AI087714, P30HD040677, S10OD010710, 1S10RR025565.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Nigora Mukhamedova
    • 1
  • Beda Brichacek
    • 2
  • Christina Darwish
    • 2
  • Anastas Popratiloff
    • 3
  • Dmitri Sviridov
    • 1
  • Michael Bukrinsky
    • 2
  1. 1.Baker IDI Heart and Diabetes InstituteMelbourneAustralia
  2. 2.Department of Microbiology, Immunology and Tropical MedicineGeorge Washington University School of Medicine and Health SciencesWashingtonUSA
  3. 3.Office of VP for ResearchGeorge Washington University Center for Microscopy and Image AnalysisWashingtonUSA

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