Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants

  • Hsuan-Chen Wu
  • Hyung Joon Cha
  • William E. Bentley
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1350)

Abstract

By use of a strategy incorporating the green fluorescent protein (GFP), facile and rapid monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two techniques for simple determination of virus titer in the baculovirus expression system using GFP co-expression and rapid monitoring of Sf-9 insect cell infection using a combination of GFP and the early-to-late (ETL) promoter of the virus vector. Because of its early appearance, GFP, when placed under the control of ETL promoter, will facilitate vector construction, virus isolation, and titer determination.

Key words

Baculovirus infection Monitoring Green fluorescent protein Insect cell culture 
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Copyright information

© Springer Science+Business Media, LLC 2016

Authors and Affiliations

  • Hsuan-Chen Wu
    • 4
    • 5
  • Hyung Joon Cha
    • 3
  • William E. Bentley
    • 1
    • 2
  1. 1.Fischell Department of BioengineeringUniversity of MarylandCollege ParkUSA
  2. 2.Institute for Bioscience and Biotechnology ResearchUniversity of MarylandCollege ParkUSA
  3. 3.Department of Chemical EngineeringPohang University of Science and TechnologyPohangKorea
  4. 4.Department of Biochemical Science and TechnologyNational Taiwan UniversityTaipeiTaiwan
  5. 5.Institute for Bioscience and Biotechnology ResearchUniversity of MarylandCollege ParkUSA

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