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A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis

  • Kenji UsuiEmail author
  • Kin-ya Tomizaki
  • Hisakazu Mihara
Part of the Methods in Molecular Biology book series (MIMB, volume 1352)

Abstract

There has been increasing interest in the potential use of microarray technologies to perform systematic and high-throughput cell-based assays. We are currently focused on developing more practical array formats and detection methods that will enable researchers to conduct more detailed analyses in cell microarray studies. In this chapter, we describe the construction of a novel peptide-array format system for analyzing cellular toxicity and cellular uptake. In this system, a peptide is immobilized at the bottom of a conventional 96-well plate using a photo-cleavable linker. The peptide can then be released from the bottom by irradiating the desired wells with UV light, thus allowing the cytotoxicity or cellular uptake of the peptide to be monitored. This system will facilitate the realization of high-throughput cell arrays for cellomics analyses and cell-based phenotypic drug screens.

Key words

Cell microarrays Designed peptide Photo-cleavable linker Cytotoxicity Cellular uptake 

Notes

Acknowledgments

We thank Mr. T. Kakiyama and Mr. T. Kikuchi (Tokyo Institute of Technology, Yokohama) for valuable discussions and generous support. This study was supported in part by grants from JSPS KAKENHI and NAGASE Science Technology Foundation. K.U. is also grateful to the JSPS KAKENHI Grant Number 26750375 from MEXT and the Grant-in-Aid for Encouragement of Young Scientists from Nakatani Foundation. K.-Y. T. acknowledges the Ryukoku University Science and Technology Fund.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.FIRST (Faculty of Frontiers of Innovative Research in Science and Technology)Konan UniversityKobeJapan
  2. 2.Department of Materials ChemistryRyukoku UniversityOtsuJapan
  3. 3.Department of Bioengineering, Graduate School of Bioscience and BiotechnologyTokyo Institute of TechnologyYokohamaJapan

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