The Purification of Natural and Recombinant Peptide Antibodies by Affinity Chromatographic Strategies

  • Hui Ma
  • Richard O’Kennedy
Part of the Methods in Molecular Biology book series (MIMB, volume 1348)


The purification of peptide antibodies (e.g., IgG, IgY, scFv, and Fab) are described in this chapter. Affinity chromatographic purification, a very convenient and effective antibody purification strategy, is used to isolate peptide antibodies based on specific binding, i.e., binding of the antibody to a column on which its specific ligand is immobilized with subsequent elution of the purified antibody. In addition, the application of purification methods based on the use of proteins A, G, and L, each of which bind to specific domains on an antibody/fragment, or the use of specific tags (e.g., histidine and biotin) attached to antibodies or antigens are also described.


IgG IgY ScFv Fab Affinity chromatographic purification Antibody–antigen binding 



This work is supported by Science Foundation Ireland under CSET Grant No. 05/CE3/B754 and 10/CE/B1821.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.School of Biotechnology and Biomedical Diagnostics InstituteDublin City UniversityDublin 9Ireland

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