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Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Amplification: An Overview

  • Joost F. SwennenhuisEmail author
  • Leon Terstappen
Part of the Methods in Molecular Biology book series (MIMB, volume 1347)

Abstract

Single cells are increasingly used to determine the heterogeneity of therapy targets in the genome during the course of a disease. The first challenge using single cells is to isolate these cells from the surrounding cells, especially when the targeted cells are rare. A number of techniques have been developed for this goal, each having specific limitations and possibilities. In this chapter, five of these techniques are discussed in the light of the isolation of circulating tumor cells (CTC) present at extremely low frequency in the blood of patients with metastatic cancer from the perspective of pre-enriched samples by means of CellSearch. The techniques described are micromanipulation, FACS, laser capture microdissection, DEPArray, and microfluidic solutions. All platforms are hampered with a low efficiency and differences in hands-on time and costs are the most important drivers for selection of the optimal platform.

Key words

CellSearch Circulating tumor cells DEPArray Laser capture microdissection Microfluidics Micromanipulation Rare cells Sample pre-enrichment Single-cell whole-genome amplification 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Medical Cell BioPhysics, Faculty of Science and Technology, MIRA Research InstituteUniversity of TwenteEnschedeThe Netherlands

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