Laser Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1™

  • Zbigniew Tadeusz Czyz
  • Nikolas H. Stoecklein
  • Bernhard PolzerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1347)


Laser microdissection (LMD) and whole genome amplification (WGA) are valuable tools to isolate, purify, and genetically analyze cancer cells from tissue sections. In this chapter, we describe a workflow for microdissecting small regions of interest from cancer tissue, i.e. formalin-fixed paraffin-embedded (FFPE) and cryo-conserved specimens, and subsequent whole genome amplification by a deterministic WGA approach (Ampli1™ WGA).

Key words

Laser microdissection (LMD) Whole genome amplification (WGA) Formalin-fixed paraffin-embedded (FFPE) tissue 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Zbigniew Tadeusz Czyz
    • 2
  • Nikolas H. Stoecklein
    • 1
  • Bernhard Polzer
    • 2
    Email author
  1. 1.Department of Visceral, General, and Pediatric SurgeryUniversity Hospital and Medical Faculty of the Heinrich-Heine University DüsseldorfDüsseldorfGermany
  2. 2.Project Group, Personalized Tumor TherapyFraunhofer Institute for Toxicology and Experimental Medicine (ITEM)RegensburgGermany

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