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Principles of Whole-Genome Amplification

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Whole Genome Amplification

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1347))

Abstract

Modern molecular biology relies on large amounts of high-quality genomic DNA. However, in a number of clinical or biological applications this requirement cannot be met, as starting material is either limited (e.g., preimplantation genetic diagnosis (PGD) or analysis of minimal residual cancer) or of insufficient quality (e.g., formalin-fixed paraffin-embedded tissue samples or forensics). As a consequence, in order to obtain sufficient amounts of material to analyze these demanding samples by state-of-the-art modern molecular assays, genomic DNA has to be amplified. This chapter summarizes available technologies for whole-genome amplification (WGA), bridging the last 25 years from the first developments to currently applied methods. We will especially elaborate on research application, as well as inherent advantages and limitations of various WGA technologies.

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Authors and Affiliations

  1. Project Group, Personalized Tumor Therapy, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Josef-Engert-Straße 9, 93053, Regensburg, Germany

    Zbigniew Tadeusz Czyz, Stefan Kirsch & Bernhard Polzer

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  1. Zbigniew Tadeusz Czyz
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  2. Stefan Kirsch
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  3. Bernhard Polzer
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Correspondence to Bernhard Polzer .

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  1. Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria

    Thomas Kroneis

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Czyz, Z.T., Kirsch, S., Polzer, B. (2015). Principles of Whole-Genome Amplification. In: Kroneis, T. (eds) Whole Genome Amplification. Methods in Molecular Biology, vol 1347. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2990-0_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2990-0_1

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  • Print ISBN: 978-1-4939-2989-4

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