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Analysis of Epithelial–Mesenchymal Transition Induced by Transforming Growth Factor β

  • Ulrich Valcourt
  • Jonathon Carthy
  • Yukari Okita
  • Lindsay Alcaraz
  • Mitsuyasu Kato
  • Sylvie Thuault
  • Laurent Bartholin
  • Aristidis Moustakas
Part of the Methods in Molecular Biology book series (MIMB, volume 1344)

Abstract

In recent years, the importance of the cell biological process of epithelial–mesenchymal transition (EMT) has been established via an exponentially growing number of reports. EMT has been documented during embryonic development, tissue fibrosis, and cancer progression in vitro, in animal models in vivo and in human specimens. EMT relates to many molecular and cellular alterations that occur when epithelial cells undergo a switch in differentiation that generates mesenchymal-like cells with newly acquired migratory and invasive properties. In addition, EMT relates to a nuclear reprogramming similar to the one occurring in the generation of induced pluripotent stem cells. Via such a process, EMT is gradually established to promote the generation and maintenance of adult tissue stem cells which under disease states such as cancer, are known as cancer stem cells. EMT is induced by developmental growth factors, oncogenes, radiation, and hypoxia. A prominent growth factor that causes EMT is transforming growth factor β (TGF-β).

A series of molecular and cellular techniques can be applied to define and characterize the state of EMT in diverse biological samples. These methods range from DNA and RNA-based techniques that measure the expression of key EMT regulators and markers of epithelial or mesenchymal differentiation to functional assays of cell mobility, invasiveness and in vitro stemness. This chapter focuses on EMT induced by TGF-β and provides authoritative protocols and relevant reagents and citations of key publications aiming at assisting newcomers that enter this prolific area of biomedical sciences, and offering a useful reference tool to pioneers and aficionados of the field.

Key words

Cellular plasticity Epithelial–mesenchymal transition Invasion Motility Signal transduction Transcription factor Transforming growth factor β 

Notes

Acknowledgements

We acknowledge funding by the Ludwig Institute for Cancer Research, the Swedish Cancer Society and the Swedish Research Council (A.M.). We also acknowledge funding by the “Ligue Nationale Contre le Cancer” (LNCC) (L.A.), the “Comité du Rhône de la Ligue Nationale Contre le Cancer” (U.V.) and the “Institut National du Cancer” (INCa) (L.B.).

We thank all past and present members of our groups for their contributions to the scientific work emanating from our laboratories. This chapter summarizes work from a selected number of published papers, however, due to space limitations, we have been unable to include all relevant publications in our discussion. We apologize to those authors whose relevant work has not been included in this review chapter.

We declare no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Ulrich Valcourt
    • 1
    • 2
    • 3
    • 4
    • 5
  • Jonathon Carthy
    • 6
  • Yukari Okita
    • 6
    • 7
  • Lindsay Alcaraz
    • 1
    • 2
    • 3
    • 4
    • 5
  • Mitsuyasu Kato
    • 7
  • Sylvie Thuault
    • 8
  • Laurent Bartholin
    • 1
    • 2
    • 3
    • 4
    • 5
  • Aristidis Moustakas
    • 6
    • 9
  1. 1.Inserm U1052, Centre de Recherche en Cancérologie de LyonLyonFrance
  2. 2.CNRS UMR 5286, Centre de Recherche en Cancérologie de LyonLyonFrance
  3. 3.Université de LyonLyonFrance
  4. 4.Université Lyon 1LyonFrance
  5. 5.Centre Léon BérardLyonFrance
  6. 6.Ludwig Institute for Cancer Research, Science for Life LaboratoryUppsala UniversityUppsalaSweden
  7. 7.Department of Experimental Pathology, Graduate School of Comprehensive Human SciencesUniversity of TsukubaTsukubaJapan
  8. 8.INSERM UMR 911 CRO2, Faculty of PharmacyMarseilleFrance
  9. 9.Department of Medical Biochemistry and Microbiology, Science for Life LaboratoryUppsala UniversityUppsalaSweden

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