Analyzing the Effect of Aging on CD8+ T-Cell Phenotype Using Flow Cytometry

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1343)

Abstract

One of the most noticeable changes in T-cell immunity with aging is the expansion of memory CD8+ T cells, with a decline in naïve phenotype T cells that reflects both diminished thymopoiesis and the effects of chronic antigenic stimulation with age. Flow cytometry is a useful tool in evaluating immune cells including the phenotype characteristics of different T-cell subsets. Here, we show flow cytometric methods measuring the different subsets of human CD8+ T cells that change with aging.

Key words

Human T cells Flow cytometry 
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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Section of Rheumatology, Department of Internal MedicineYale School of MedicineNew HavenUSA

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