Abstract
The eukaryotic cell cycle is comprised of different phases that take place sequentially once, and normally only once, every division cycle. Such a dynamic process is best viewed in real time in living dividing cells. The insights that can be gained from such methods are considerably larger than any alternative technique that only generates snapshots. A great number of studies can gain from live cell imaging; however this method often feels somewhat intimidating to the novice. The purpose of this chapter is to demonstrate that imaging cell cycle phases in living cells from yeast to human is relatively easy and can be performed with equipment that is available in most research institutes. We present the different approaches, review different types of reporters, and discuss in depth all the aspects to be considered to obtain optimal results. We also describe our latest cell cycle markers, which afford unprecedented “sub”-phase temporal resolution.
Using a title from yeast to man is not only chauvinistic but also wrong—all the human cells we used for this work—HeLa, HT1080 U2OS, and RPE1—are female cells.
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Acknowledgements
We would like to thank Aryeh Weiss, Amit Zur, Tamar Listovsky, Yuval Cinnamon, Julia Sajman, and the many other students and colleagues who helped to develop and practice live cell imaging in our laboratory over the last two decades. Special thanks to Tim Hunt who suggested that I should use live cell imaging in the first place. This research in our laboratory was funded over the years by grants from the ISF, BSF, GIF, AICR, ICRF, and HUJI.
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Segev, H., Zenvirth, D., Simpson-Lavy, K.J., Melamed-Book, N., Brandeis, M. (2016). Imaging Cell Cycle Phases and Transitions of Living Cells from Yeast to Woman. In: Coutts, A., Weston, L. (eds) Cell Cycle Oscillators. Methods in Molecular Biology, vol 1342. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2957-3_20
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DOI: https://doi.org/10.1007/978-1-4939-2957-3_20
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2956-6
Online ISBN: 978-1-4939-2957-3
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