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Building a Synthetic Transcriptional Oscillator

  • Matthaeus Schwarz-Schilling
  • Jongmin Kim
  • Christian Cuba
  • Maximilian Weitz
  • Elisa Franco
  • Friedrich C. SimmelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1342)

Abstract

Reaction circuits mimicking genetic oscillators can be realized with synthetic, switchable DNA genes (so-called genelets), and two enzymes only, an RNA polymerase and a ribonuclease. The oscillatory behavior of the genelets is driven by the periodic production and degradation of RNA effector molecules. Here, we describe the preparation, assembly, and testing of a synthetic, transcriptional two-node negative-feedback oscillator, whose dynamics can be followed in real-time by fluorescence read-out.

Key words

Gene oscillator Transcription circuit In vitro Nonlinear dynamics Negative-feedback oscillator Genelet circuits 

Notes

Acknowledgement

This work was supported by the Deutsche Forschungsgemeinschaft through the Cluster of Excellence Nanosystems Initiative Munich (NIM) and by NSF grants CMMI-1266402 and CCF-131729.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Matthaeus Schwarz-Schilling
    • 1
  • Jongmin Kim
    • 2
  • Christian Cuba
    • 3
  • Maximilian Weitz
    • 1
  • Elisa Franco
    • 3
  • Friedrich C. Simmel
    • 1
    Email author
  1. 1.Systems Biophysics and Bionanotechnology, Physik Department and WSI/ZNNTechnische Universität MünchenGarchingGermany
  2. 2.Wyss Institute for Biologically Inspired EngineeringHarvard UniversityBostonUSA
  3. 3.Department of Mechanical EngineeringUniversity of California, RiversideRiversideUSA

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