Building a Synthetic Transcriptional Oscillator
Reaction circuits mimicking genetic oscillators can be realized with synthetic, switchable DNA genes (so-called genelets), and two enzymes only, an RNA polymerase and a ribonuclease. The oscillatory behavior of the genelets is driven by the periodic production and degradation of RNA effector molecules. Here, we describe the preparation, assembly, and testing of a synthetic, transcriptional two-node negative-feedback oscillator, whose dynamics can be followed in real-time by fluorescence read-out.
Key wordsGene oscillator Transcription circuit In vitro Nonlinear dynamics Negative-feedback oscillator Genelet circuits
This work was supported by the Deutsche Forschungsgemeinschaft through the Cluster of Excellence Nanosystems Initiative Munich (NIM) and by NSF grants CMMI-1266402 and CCF-131729.
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