Mesenchymal Stem Cells Derived from Human Bone Marrow

  • Oliver F. W. Gardner
  • Mauro Alini
  • Martin J. StoddartEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1340)


Mesenchymal stem cells are found in a number of tissues and have the potential to differentiate into a range of mesenchymal lineages. This ready availability and multipotent character means that mesenchymal stem cells have become a focus for the field of tissue engineering, particularly for the repair of bone and cartilage.

This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the cells and characterisation of their multipotency.

Key words

Mesenchymal stem cell Osteogenesis Chondrogenesis Adipogenesis 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Oliver F. W. Gardner
    • 1
    • 2
  • Mauro Alini
    • 1
  • Martin J. Stoddart
    • 1
    Email author
  1. 1.Musculoskeletal RegenerationAO Research Institute DavosDavos PlatzSwitzerland
  2. 2.Cardiff University School of BiosciencesCardiffUK

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