Mesenchymal Stem Cells Derived from Human Bone Marrow
Mesenchymal stem cells are found in a number of tissues and have the potential to differentiate into a range of mesenchymal lineages. This ready availability and multipotent character means that mesenchymal stem cells have become a focus for the field of tissue engineering, particularly for the repair of bone and cartilage.
This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the cells and characterisation of their multipotency.
Key wordsMesenchymal stem cell Osteogenesis Chondrogenesis Adipogenesis
- 4.Friedenstein AJ, Gorskaja JF, Kulagina NN (1976) Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 4:267–274Google Scholar
- 5.Friedenstein AJ, Chailakhjan RK, Lalykina KS (1970) The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 3:393–403Google Scholar