Protein Arginylation pp 79-82 | Cite as
High-Throughput Arginylation Assay in Microplate Format
Protocol
Abstract
Here we describe the biochemical assay for ATE1-mediated arginylation in microplate format, which can be applied to high-throughput screens for identification of small-molecule inhibitors and activators of ATE1, high-volume analysis of ATE1 substrates, and other similar applications. Originally, we have applied this screen to a library of 3280 compounds and identified two compounds which specifically affect ATE1-regulated processes in vitro and in vivo. The assay is based on in vitro ATE1-mediated arginylation of beta-actin’s N-terminal peptide, but it can also be applied using other ATE1 substrates.
Key words
ATE1 inhibitors Arginylation Tannic acid Cell motility AngiogenesisReferences
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