Genome Editing in Mice Using TALE Nucleases
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
Key wordsKnock-in Knockout One-cell embryo Gene targeting TALEN Mouse
This work was supported by the European Union within the EUCOMM project (LSHG-CT-2005-018931, to W.W.), by the German Ministry of Education and Research within the TAL-CUT (03 V0261) and DIGTOP project (01GS0858) (to W.W. and R.K.) of the VIP and NGFN-Plus program.