TALENs pp 229-243 | Cite as

Genome Editing in Mice Using TALE Nucleases

  • Benedikt Wefers
  • Christina Brandl
  • Oskar Ortiz
  • Wolfgang WurstEmail author
  • Ralf KühnEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1338)


Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.

Key words

Knock-in Knockout One-cell embryo Gene targeting TALEN Mouse 



This work was supported by the European Union within the EUCOMM project (LSHG-CT-2005-018931, to W.W.), by the German Ministry of Education and Research within the TAL-CUT (03 V0261) and DIGTOP project (01GS0858) (to W.W. and R.K.) of the VIP and NGFN-Plus program.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institute of Developmental GeneticsHelmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany
  2. 2.Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE)MunichGermany
  3. 3.Technische Universität MünchenFreising-WeihenstephanGermany
  4. 4.Max Planck Institute of PsychiatryMunichGermany
  5. 5.Munich Cluster for Systems Neurology (SyNergy)Ludwig-Maximilians-Universität MünchenMunichGermany
  6. 6.Max-Delbrück-Center for Molecular MedicineBerlinGermany
  7. 7.Berlin Institute of HealthBerlinGermany

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