Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages

  • Inés Pineda-Torra
  • Matthew Gage
  • Alba de Juan
  • Oscar M. Pello
Part of the Methods in Molecular Biology book series (MIMB, volume 1339)


Macrophages are the most specialized phagocytic cells, and acquire specific phenotypes and functions in response to a variety of external triggers. Culture of bone marrow-derived or peritoneal macrophages from mice represents an exceptionally powerful technique to investigate macrophage phenotypes and functions in response to specific stimuli, resembling as much as possible the conditions observed in various pathophysiological settings. This chapter outlines protocols used to isolate and culture murine bone marrow-derived and peritoneal macrophages. Furthermore, we describe how these macrophages can be “polarized” to obtain specific macrophage subsets with special relevance to atherosclerosis.

Key words

Bone marrow-derived macrophages Peritoneal macrophages Macrophage polarization Atherosclerosis 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Division of Medicine, Centre for Clinical PharmacologyUniversity College LondonLondonUK
  2. 2.Walter Brendel Center for Experimental MedicineLudwig-Maximilians-UniversitätMunichGermany
  3. 3.Department of Hematology, John Goldman Centre for Cellular TherapyHammersmith HospitalLondonUK

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