In Vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis
Positron emission tomography (PET) is an important technique in cardiovascular research. Vascular inflammation detected by fluorodeoxyglucose (FDG)-PET has been shown to predict cardiovascular (CV) events independent of traditional risk factors and is also highly associated with overall burden of atherosclerosis. The use of PET imaging in mouse models of atherosclerosis is challenged by the reduced size of the scanned organs. However, the last generation of dedicated PET scanners has an improved spatial resolution (<1 mm) and increased sensitivity allowing those studies to be performed. Here, we describe a procedure to perform FDG-PET experiments in atherosclerosis mouse models, the required equipment for animal handling and imaging, and the tools and procedures for image analysis and validation of the results.
Key wordsPositron emission tomography (PET) Fluorodeoxyglucose (FDG) Computed tomography (CT) Vascular imaging Atherosclerosis Small animal imaging
JJ Vaquero is partially funded by the Cardiovascular Research Network (RIC, RD12/0042/0057) from the Ministerio de Economía y Competitividad (www.mineco.gob.es/). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.