Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array

  • Ryota Iino
  • Shouichi Sakakihara
  • Yoshimi Matsumoto
  • Kunihiko Nishino
Part of the Methods in Molecular Biology book series (MIMB, volume 1333)

Abstract

A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.

Keywords

Microfabrication Microdevice Microdroplet array Single-cell analysis Drug tolerance Persister Pseudomonas aeruginosa Optical microscopy Micropipette 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Ryota Iino
    • 1
    • 2
  • Shouichi Sakakihara
    • 3
  • Yoshimi Matsumoto
    • 4
  • Kunihiko Nishino
    • 4
  1. 1.Okazaki Institute for Integrative Bioscience, Institute for Molecular Science, National Institutes of Natural SciencesAichiJapan
  2. 2.Department of Functional Molecular Science, School of Physical SciencesThe Graduate University for Advanced Studies (SOKENDAI)KanagawaJapan
  3. 3.Technical Division, Institute of Scientific and Industrial ResearchOsaka UniversityOsakaJapan
  4. 4.Laboratory of Microbiology and Infectious Diseases, Institute of Scientific and Industrial ResearchOsaka UniversityOsakaJapan

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