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Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy

  • Thomas R. Hurd
  • Carlos G. Sanchez
  • Felipe K. Teixeira
  • Chris Petzold
  • Kristen Dancel-Manning
  • Ju-Yu S. Wang
  • Ruth Lehmann
  • Feng-Xia A. LiangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1328)

Abstract

The Drosophila melanogaster ovary is a powerful, genetically tractable system through which one can elucidate the principles underlying cellular function and organogenesis in vivo. In order to understand the intricate process of oogenesis at the subcellular level, microscopic analysis with the highest possible resolution is required. In this chapter, we describe the preparation of ovaries for ultrastructural analysis using transmission electron microscopy and focused ion beam scanning electron microscopy. We discuss and provide protocols for chemical fixation of Drosophila ovaries that facilitate optimal imaging with particular attention paid to preserving and resolving mitochondrial membrane morphology and structure.

Keywords

Drosophila ovary Germline Transmission electron microscopy (TEM) Mitochondria Cristae Focused ion beam scanning electron microscopy (FIB-SEM) 

Notes

Acknowledgements

We would like to thank NYULMC OCS for the support to Microscopy Core, Edward Eng and William Rice from New York Structure Biology Center for the help of FIB-SEM imaging. TRH was supported by CIHR; FKT by EMBO and HFSP long-term fellowships; CS by NIH F31/HD080380. RL is an HHMI investigator and supported by NIH R01/R37HD41900.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Thomas R. Hurd
    • 1
    • 2
    • 3
  • Carlos G. Sanchez
    • 1
    • 2
    • 3
  • Felipe K. Teixeira
    • 1
    • 2
    • 3
  • Chris Petzold
    • 4
  • Kristen Dancel-Manning
    • 4
  • Ju-Yu S. Wang
    • 1
    • 2
    • 3
  • Ruth Lehmann
    • 1
    • 2
    • 3
  • Feng-Xia A. Liang
    • 3
    • 4
    Email author
  1. 1.Howard Hughes Medical Institute (HHMI)New York University School of MedicineNew YorkUSA
  2. 2.Kimmel Center for Biology and Medicine of the Skirball InstituteNew York University School of MedicineNew YorkUSA
  3. 3.Department of Cell BiologyNew York University School of MedicineNew YorkUSA
  4. 4.Office of Collaborative Science Microscopy CoreNew York University School of MedicineNew YorkUSA

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