C. elegans pp 209-219 | Cite as

A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans Dauer Larvae

  • Maria C. Ow
  • Sarah E. HallEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1327)


The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purified dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals.

Key words

Caenorhabditis elegans Pheromone Stress Dauer Diapause Postdauer Egg white plate 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of BiologySyracuse UniversitySyracuseUSA

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