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C. elegans pp 209-219 | Cite as

A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans Dauer Larvae

  • Maria C. Ow
  • Sarah E. HallEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1327)

Abstract

The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purified dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals.

Key words

Caenorhabditis elegans Pheromone Stress Dauer Diapause Postdauer Egg white plate 

References

  1. 1.
    Cassada RC, Russell RL (1975) The dauer larva, a postembryonic developmental variant of the nematode Caenorhabditis elegans. Dev Biol 46:326–342CrossRefPubMedGoogle Scholar
  2. 2.
    Golden JW, Riddle DL (1984) The Caenorhabditis elegans dauer larva: developmental effects of pheromone, food, and temperature. Dev Biol 102:368–378CrossRefPubMedGoogle Scholar
  3. 3.
    Riddle DL (1997) C. elegans II. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, pp 739–768Google Scholar
  4. 4.
    Hu, PJ (2007) Dauer WormBook. In: The C. elegans Research Community (ed) WormBook, doi:  10.1895/wormbook.1.144.1, http://www.wormbook.org
  5. 5.
    Albert PS, Riddle DL (1983) Developmental alterations in sensory neuroanatomy of the Caenorhabditis elegans dauer larva. J Comp Neurol 219:461–481CrossRefPubMedGoogle Scholar
  6. 6.
    Cherkasova V, Ayyadevara S, Egilmez N, Shmookler Reis R (2000) Diverse Caenorhabditis elegans genes that are upregulated in dauer larvae also show elevated transcript levels in long-lived, aged, or starved adults. J Mol Biol 300:433–448CrossRefPubMedGoogle Scholar
  7. 7.
    Jones SJ, Riddle DL, Pouzyrev AT, Velculescu VE, Hillier L, Eddy SR, Stricklin SL, Baillie DL, Waterston R, Marra MA (2001) Changes in gene expression associated with developmental arrest and longevity in Caenorhabditis elegans. Genome Res 11:1346–1352CrossRefPubMedGoogle Scholar
  8. 8.
    Wang J, Kim SK (2003) Global analysis of dauer gene expression in Caenorhabditis elegans. Development 130:1621–1634CrossRefPubMedGoogle Scholar
  9. 9.
    Fielenbach N, Antebi AC (2008) C. elegans dauer formation and the molecular basis of plasticity. Genes Dev 22:2149–2165PubMedCentralCrossRefPubMedGoogle Scholar
  10. 10.
    Hall SE, Beverly M, Russ C, Nusbaum C, Sengupta P (2010) A cellular memory of developmental history generates phenotypic diversity in C. elegans. Curr Biol 20:149–155PubMedCentralCrossRefPubMedGoogle Scholar
  11. 11.
    Procko C, Lu Y, Shaham S (2011) Glia delimit shape changes of sensory neuron receptive endings in C. elegans. Development 138:1371–1381PubMedCentralCrossRefPubMedGoogle Scholar
  12. 12.
    Karp X, Hammell M, Ow MC, Ambros V (2011) Effect of life history on microRNA expression during C. elegans development. RNA 17:639–651PubMedCentralCrossRefPubMedGoogle Scholar
  13. 13.
    Procko C, Lu Y, Shaham S (2012) Sensory organ remodeling in Caenorhabditis elegans requires the zinc-finger protein ZTF-16. Genetics 190:1405–1415PubMedCentralCrossRefPubMedGoogle Scholar
  14. 14.
    Hall SE, Chirn GW, Lau NC, Sengupta P (2013) RNAi pathways contribute to developmental history-dependent phenotypic plasticity in C. elegans. RNA 19:306–319PubMedCentralCrossRefPubMedGoogle Scholar
  15. 15.
    Schroeder NE, Androwski RJ, Rashid A, Lee H, Lee J, Barr MM (2013) Dauer-specific dendrite arborization in C. elegans is regulated by KPC-1/Furin. Curr Biol 19:1527–1535CrossRefGoogle Scholar
  16. 16.
    Billi AC, Fischer SE, Kim JK (2014) Endogenous RNAi pathways in C. elegans WormBook. In: The C. elegans Research Community (ed) WormBook, doi:  10.1895/wormbook.1.170.1, http://www.wormbook.org
  17. 17.
    Wedeles CJ, Wu MZ, Claycomb JM (2013) A multitasking Argonaute: exploring the many facets of C. elegans CSR-1. Chromosome Res 21:573–586CrossRefPubMedGoogle Scholar
  18. 18.
    González-Aguilera C, Palladino F, Askjaer P (2014) C. elegans epigenetic regulation in development and aging. Brief Funct Genomics 13:223–234PubMedCentralCrossRefPubMedGoogle Scholar
  19. 19.
    Guérin TM, Palladino F, Robert VJ (2014) Transgenerational functions of small RNA pathways in controlling gene expression in C. elegans. Epigenetics 9:37–44PubMedCentralCrossRefPubMedGoogle Scholar
  20. 20.
    Lewis JA, Fleming JT (1995) Basic culture methods. In: Epstein HF, Shakes DC (eds) Methods in cell biology. Caenorhabditis elegans: modern biological analysis of an organism. Academic Press, Inc., San Diego, pp 4–27Google Scholar
  21. 21.
    Butcher RA, Fujita M, Schroeder FC, Clardy J (2007) Small-molecule pheromones that control dauer development in Caenorhabditis elegans. Nat Chem Biol 3:420–422CrossRefPubMedGoogle Scholar
  22. 22.
    Butcher RA, Ragains JR, Kim E, Clardy J (2008) A potent dauer pheromone component in Caenorhabditis elegans that acts synergistically with other components. Proc Natl Acad Sci U S A 105:14288–14292PubMedCentralCrossRefPubMedGoogle Scholar
  23. 23.
    Stiernagle T (2006) Maintenance of C. elegans, WormBook. In: The C. elegans Research Community (ed) WormBook, doi:  10.1895/wormbook.1.101.1, http://www.wormbook.org
  24. 24.
    Portman DS (2006) Profiling C. elegans gene expression with DNA microarrays, WormBook. In: The C. elegans Research Community (ed) WormBook, doi:  10.1895/wormbook.1.104.1, http://www.wormbook.org

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of BiologySyracuse UniversitySyracuseUSA

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