Flow Cytometry Analysis of Thymic Epithelial Cells and Their Subpopulations

  • Izumi Ohigashi
  • Yousuke TakahamaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1323)


The parenchyma of the thymus is compartmentalized into the cortex and the medulla, which are constructed by cortical thymic epithelial cells (cortical TECs, cTECs) and medullary thymic epithelial cells (mTECs), respectively. cTECs and mTECs essentially and differentially regulate the development and repertoire selection of T cells. Consequently, the biology of T cell development and selection includes the study of TECs in addition to the study of developing T cells and other hematopoietic cells including dendritic cells. In this chapter, we describe the methods for flow cytometric analysis and sorting of TECs and their subpopulations, including cTECs and mTECs.

Key words

Thymic epithelial cell mTEC cTEC Aire β5t Flow cytometry Cell sorting 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Division of Experimental Immunology, Institute for Genome ResearchUniversity of TokushimaTokushimaJapan

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