Determining the Effects of Membrane-Interacting Peptides on Membrane Integrity

Part of the Methods in Molecular Biology book series (MIMB, volume 1324)


In the study of cell-penetrating and membrane-translocating peptides, a fundamental question occurs as to the contribution arising from fundamental peptide–membrane interactions, relative to the contribution arising from the biology and energy of the cell, mostly occurring in the form of endocytosis and subsequent events. A commonly used approach to begin addressing these mechanistic questions is to measure the degree to which peptides can interact with, and physically disrupt, the integrity of synthetic lipid bilayers. Here, we describe a set of experimental methods that can be used to measure the potency, kinetics, transience, and the effective size of peptide-induced membrane disruption.

Key words

Vesicle leakage Bilayer permeabilization Pore formation Leakage assay Terbium ANTS DPX Transient leakage Pore size 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular BiologyTulane University School of MedicineNew OrleansUSA

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