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Determination and Analysis of Cellular Metabolic Changes by Noncanonical Hedgehog Signaling

  • Raffaele Teperino
  • John Andrew Pospisilik
Part of the Methods in Molecular Biology book series (MIMB, volume 1322)

Abstract

Hedgehog is a morphogen essential for body patterning and proper embryonic development from flies to humans. Thought quiescent in adults, its inappropriate reactivation is associated with many disparate genetic and sporadic types of human cancers. Recent findings have demonstrated a key, yet unexpected, role of the Hedgehog signaling pathway in metabolic control. Here, we describe a panel of methods to determine and analyze cellular and organismal metabolic changes downstream of the Hedgehog signaling pathway.

Key words

Hedgehog Metabolism Glucose uptake Calcium Ampk 

Notes

Acknowledgements

JAP acknowledges generous support from the DFG, ERC, EU-FP7, BMBF (DEEP), and the MPG.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Institute of Experimental Genetics, Helmholtz Zentrum MünchenGerman Research Center for Environmental HealthNeuherberg, MunichGermany
  2. 2.Max Planck Institute of Immunobiology and EpigeneticsFreiburg im BreisgauGermany

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