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Yeast Surface Display

Volume 1319 of the series Methods in Molecular Biology pp 155-175

Applications of Yeast Surface Display for Protein Engineering

  • Gerald M. CherfAffiliated withDepartment of Bioengineering, Stanford University
  • , Jennifer R. CochranAffiliated withDepartment of Bioengineering, Stanford UniversityDepartment of Chemical Engineering, Stanford University Email author 

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Abstract

The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. Additional applications of yeast display are also briefly presented, including protein epitope mapping, identification of protein-protein interactions, and uses of displayed proteins in industry and medicine.

Key words

Yeast surface display Protein engineering Random mutagenesis DNA shuffling Affinity maturation Protein stability engineering Enzyme engineering