MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine for Cancer Therapy

  • Manuel Schmidt
  • Barbara Volz
  • Patrick Großmann
  • Kerstin Heinrich
  • Burghardt Wittig
Part of the Methods in Molecular Biology book series (MIMB, volume 1317)


Gene modification of eukaryotic cells by electroporation is a widely used method to express selected genes in a defined cell population for various purposes, like gene correction or production of therapeutics. Here, we describe the generation of a cell-based tumor vaccine via fourfold transient gene modification of a human renal cell carcinoma (RCC) cell line for high expression of CD80, CD154, GM-CSF, and IL-7 by use of MIDGE® vectors. The two co-stimulatory molecules CD80 and CD154 are expressed at the cell surface, whereas the two cytokines GM-CSF and IL-7 are secreted yielding cells with enhanced immunological properties. These fourfold gene-modified cells have been used as a cell-based tumor vaccine for the treatment of RCC.

Key words

Cell-based tumor vaccine MIDGE vector Non-viral vectors Electroporation Transient transfection 



We thank Florian Sack for expert review of the manuscript.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Mologen AG BerlinBerlinGermany
  2. 2.Mologen AGBerlinGermany
  3. 3.Foundation Institute Molecular Biology and BioinformaticsFreie Universität BerlinBerlinGermany

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