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Parallel Donor Genotyping for 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput MALDI-TOF Mass Spectrometry

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1310)

Abstract

Most blood group antigens are defined by single nucleotide polymorphisms (SNPs). Highly accurate MALDI-TOF MS has proven its potential in SNP genotyping and was therefore chosen for blood donor oriented genotyping with high-throughput capability, e.g., 380 samples per day. The Select Module covers a total of 36 SNPs in two single-tube reactions, representative of 46 blood group and 4 human platelet antigens. Using this tool, confirmatory blood group typing for RhD, RhCE, Kell, Kidd, Duffy, MN, Ss, and selected rare antigens is performed on a routine basis.

Key words

Blood group gene Blood group genotyping High-throughput SNP typing MALDI-TOF MS Kell Kidd Duffy MNSs Rare blood group antigens Blood donor 

Notes

Acknowledgements

Financial support for the development of the Select Module was granted by the Humanitarian Foundation of the Swiss Red Cross (SRC); the Blood Transfusion Service Zurich, SRC, Switzerland and the Swiss blood transfusion umbrella organization Blutspende SRK Schweiz, Bern, Switzerland. SM, NT, and BMF are employees of the Blood Transfusion Service Zurich, SRC, Switzerland, and have not disclosed any conflicts of interest. CG is employee of the Blood Transfusion Service Zurich, SRC, Switzerland, and acts as a consultant for Inno-Train GmbH, Kronberg im Taunus, Germany.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Molecular Diagnostics and Research (MOC)Swiss Red Cross Blood Transfusion Service ZurichSchlierenSwitzerland

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