Molecular Typing of Blood Cell Antigens pp 247-258 | Cite as
In Silico HLA Typing Using Standard RNA-Seq Sequence Reads
Protocol
Abstract
Next-generation sequencing (NGS) enables high-throughput transcriptome profiling using the RNA-Seq assay, resulting in billions of short sequence reads. Worldwide adoption has been rapid: many laboratories worldwide generate transcriptome sequence reads daily. Here, we describe methods for obtaining a sample’s human leukocyte antigen (HLA) class I and II types and HLA expression using standard NGS RNA-Seq sequence reads. We demonstrate the application using our algorithm, seq2HLA, and a publicly available RNA-Seq dataset from the Burkitt lymphoma cell line Raji.
Key words
HLA type HLA expression NGS RNA-Seq Immunoinformatics In silicoReferences
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