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Validation of Genotyping Protocols for Diagnostic Use

  • Paul MetcalfeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1310)

Abstract

This chapter contains some advice for first validating a new genotyping method and then continuing to ensure that it performs well when adopted for routine use. It should be applicable to most of the genotyping methods described in this volume; however, because the range of techniques is so wide and these recommendations have been written with PCR amplification in mind, the reader may need to adapt some of the recommendations for non-PCR based tests.

It does not include any advice on how to handle unscreened human samples within the laboratory, and it assumes that the laboratory already has established good practice for DNA amplification and the avoidance of contamination.

Key words

Genotyping Protocol Validation 

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.National Institute for Biological Standards and ControlHertsUK

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