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Cortical Differentiation of Human Pluripotent Cells for In Vitro Modeling of Alzheimer’s Disease

  • Nathalie G. Saurat
  • Frederick J. LiveseyEmail author
  • Steven Moore
Part of the Methods in Molecular Biology book series (MIMB, volume 1303)

Abstract

Stem cell models of Alzheimer’s disease provide an opportunity to study the mechanisms underlying disease pathology at a resolution that is not possible in animal models. Furthermore, the ability to reprogram patient somatic cells to a pluripotent state ensures that the disease can be investigated in the correct genetic context. Here, we describe the directed differentiation of human pluripotent cells to cortical progenitors by recapitulating key developmental signaling events in vitro. Over a timeframe that mirrors human development, these progenitors give rise to functional lower and upper layer neurons. We also describe biochemical and imaging based methods to analyse key APP and Tau phenotypes in neurons generated from pluripotent stem cells from individuals with either monogenic familial Alzheimer’s disease or Down’s syndrome.

Key words

Disease modeling Alzheimer’s disease Stem cell APP Abeta peptides Tau Cortical differentiation Neurodegeneration Down’s syndrome 

Notes

Acknowledgements

Work in the lab of FJL is supported by the Wellcome Trust, Alzheimer’s Research UK, the Medical Research Council and the EU Innovative Medicines Initiative StemBANCC. NS is supported by the Woolf Fisher Trust. FJL is a Wellcome Trust Senior Investigator.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Nathalie G. Saurat
    • 1
    • 2
    • 3
  • Frederick J. Livesey
    • 1
    • 2
    • 3
    Email author
  • Steven Moore
    • 1
    • 2
    • 3
  1. 1.Cambridge Stem Cell InstituteUniversity of CambridgeCambridgeUK
  2. 2.Department of BiochemistryUniversity of CambridgeCambridgeUK
  3. 3.Gurdon InstituteUniversity of CambridgeCambridgeUK

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